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In this paper, we performed a comparative blind study for Salmonella enterica typing, comparing side by side standard processing and Magelia processing. PCR-free library preparation was used for blind NGS typing of this relevant foodborne pathogen, for samples ranging from 5 ng to 85 ng.
Our multi-omics platform Magelia challenges the minimum required input for a PCR-free library kit, by reducing reagent volumes 8x and DNA input 50x while yielding high quality sequencing libraries.