Our partner GCS SeqOIA from the French National Genomic Plan (PFMG2025) is presenting a poster showcasing their transcriptomic workflows automated on the Magelia.
Extensive molecular characterisation of cancer tissues is essential for precision medicine, and in that context:
Sequencing strategies are crucial
Whole Transcriptome profiling (WTS) is of major interest for the detection of
fusion transcripts
gene expression levels
expression signatures, tissue of origin...
WTS library preparation protocols must capture all molecules of interest, excluding rRNA, regardless of the quality of total RNA available
Small size of tumor biopsy is sometimes a limitation
Automated solutions for library preparation are necessary to increase throughput, reduce turnaround time, lower costs, ensure high reproducibility, and minimize the risk of contamination.
PFMG SeqOIA lab evaluated the Magelia® (Inorevia) automated platform for WTS with a range of RNA inputs, in order to validate a rapid and robust workflow for technically challenging samples.
Overall, wetlab operations have significantly gained efficiency with Magelia® system: launching overnight runs requires minimal hands-on time, automation drastically reduces the risks of error and cross-contamination; optimized turnaround time now makes it possible to sequence urgent samples sequenceable on the second day after sample reception. These data highlight improved transcriptome resolution for Magelia®-treated samples.